Keratinocyte, which is a major component of the epidermis, maintains and repairs the tissues by the production of such substances as hyaluronic acid. It has been reported that this process is affected by immune cells in the skin, and if a wound occurs, the immune cells induce the production of hyaluronic acid from the keratinocytes to promote wound healing. HK L-137 is expected to regulate hyaluronic acid production from keratinocytes if it acts on immune cells in the skin through its immunostimulating activity.
In this study, the effect of HK L-137 on the production of hyaluronic acid from keratinocytes was examined by adding HK L-137 to the epidermal cell culture that contain keratinocytes and immune cells. As a result, increases in the production of hyaluronic acid were observed by the addition of HK L-137, indicating that HK L-137 may be effective to improve skin functions.
Epidermal cells from the skin of mice were cultured for 7 days and the concentrations of hyaluronic acid in the culture medium were measured. HK L-137 was added to the culture medium at the concentration of 1 µg/ml.
The addition of HK L-137 increased the production of hyaluronic acid from keratinocytes.
Cultured epidermal cells contain Langerhans cells and other immune cells specific for the epidermal tissues. HK L-137 may act on these cells to produce some factors that induce the production of hyaluronic acid from keratinocytes. It is also possible that HK L-137 directly acts on keratinocytes. In association with the observation, in vivo study shows that the moisture in the skin was kept in mice given HK L-137 orally.*
These results indicate that HK L-137 may control the functions of the skin by enhancing immune functions.
* Reference: Patent, “Moisturizing agent” WO2008/018143